Fraunhofer: low-energy-electrons for different approach to vaccine production

January 4, 2017
Most inoculations are based on inactivated vaccines, containing viruses that have been killed and whose pathogens can no longer harm recipients. The immune system nevertheless recognizes them, produces the necessary antibodies, and therefore provides effective protection. Up to now, chemicals – typically formaldehyde – have been used to kill the pathogens.

But there are several disadvantages to that method: formaldehyde, just like the other chemicals used for the same purpose, is toxic. To minimize the risks to human health and the environment, these substances are used only when greatly diluted. This means that the pathogens must be exposed to the chemical for a long time in order to be destroyed.

For instance, formaldehyde takes around two weeks to inactivate the poliovirus, which triggers poliomyelitis. This long proces represents a disadvantage for industry. Moreover, formaldehyde also modifies the proteins in the viruses against which the immune system produces antibodies, altering . the viruses and in doing so,  reducing the effectiveness of the vaccine.

Promising alternative

Researchers at the Fraunhofer Institutes for Cell Therapy and Immunology IZI, for Interfacial Engineering and Biotechnology IGB, for Organic Electronics, Electron Beam and Plasma Technology FEP, and for Manufacturing Engineering and Automation IPA, have now come up with a promising alternative.

 “We use low-energy electrons to irradiate the pathogens,” says Dr. Sebastian Ulbert, head of the working group at Fraunhofer IZI. Rather than days or even weeks, only a few milliseconds are needed to kill off viruses or bacteria. Not only does this significantly shorten vaccine production times, the electrons destroy only the nucleic acids in the viruses and bacteria, leaving their proteins intact. So the elements to which our immune system launches the desired immune response are also still intact after irradiation. A further important benefit is that no toxic chemicals are produced.

Irradiation experiments

While there have long been experiments to use irradiation to eliminate pathogens, the experimental effort required has so far proven virtually unmanageable. For safety reasons, exposure to radioactive irradiation was possible only behind solid walls – certainly not within the production halls of the pharmaceutical industry.

Low-energy electron irradiation, however, is possible in a normal laboratory. At the laboratory scale of 10 to 15 milliliters, researchers have already shown that the technique is error free: viruses are verifiably eliminated, and in initial experiments on animal models, the vaccine proved comprehensive protection.

Thin layers

In a next step, the scientists want to inactivate viruses in larger volumes. Since low-energetic electrons penetrate less than a millimeter into the liquid containing the viruses, the liquid must be presented in thin layers if the electrons are to reach every last target.

With funding from the Bill & Melinda Gates Foundation, researchers are now developing two suitable prototypes that will inactivate pathogens automatically. The first prototype is almost finished: the solution to be irradiated is filled into bags, which ensures a sufficiently thin layer of liquid. With the second prototype, the scientists achieve a fine layer of liquid by running the solution over rollers. The scientists hope that clinical trials for vaccine production using these methods can start in around five years’ time.

The applications of the new technology are not restricted to vaccines. “Using electron irradiation, we can also inactivate hazardous material without destroying it,” says Dr. Ulbert. For example, this  would allow blood samples taken from people infected with the Ebola virus to be prepared in such a way that they can be examined safely in ordinary laboratories.

Working together

Four Fraunhofer Institutes have pooled their expertise to work on viral inactivation. Fraunhofer IZI delivered proof of concept, which means it has demonstrated that the technique works as intended. Its researchers also undertook the vaccination studies. Colleagues at Fraunhofer IGB molecularly characterize the effect of irradiation on pathogens. Pathogens were inactivated due to the destruction of their genomes. At the same time, pathogen proteins were preserved enabling their use for efficient vaccination.

Fraunhofer FEP is providing the irradiation technology, contributing its expertise in dosimetry, and is involved in building the prototype that is to be housed at Fraunhofer IZI in Leipzig. Fraunhofer IPA is developing a completely new technical procedure to transfer the "laboratory scale inactivation of small volumes" to an industrial scale for vaccine production.